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Welcome to
the
London Regional Genomics Centre
The London Regional Genomics Centre is a Robarts Research Institute led initiative and has been established through generous support from the Robarts Research Institute, The Schulich School of Medicine and Dentistry at the University of Western Ontario, London Health Sciences Centre, Child Health Research Institute and The London Regional Cancer Program. The facility provides services on a fee-for-service/cost recovery basis and offers Affymetrix GeneChip technologies and NGS services on the Ion Torrent and MiSeq platforms.
Download: Sequence Alignment software comparison
Download: Pathway Analysis software comparison
Download Poster: LRGC Services
Download
Poster: LRGC Data QC
Latest News
User's Survey (Please email or Fax back to us)
User's Published Papers (128) (updated November 21, 2013)
Ingenuity Pathway Analysis Online Training
The LRGC now offers Affymetrix CytoScan HD Array
The LRGC now offers Affymetrix Human Transcriptome Array 2.0
The LRGC now offers 2 x 250 bp PE reads on the Illumina MiSeq
The LRGC now offers NGS services on the Illumina MiSeq
The LRGC is now an official Ion Torrent Certified Service Provider
Now offering the Affymetrix miRNA 3.0 Array
Now offering Partek Pathway
Now offering microbiome sequencing Please contact
Now offering the Affymetrix Mouse Diversity Genotyping Array
Now offering Ion Torrent Next-Generation Sequencing
Now offering DNA fragmentation using the Covaris S2
Partek Data Analysis Workshop - Slides: NGS and Microarray
Other services: AB ViiA7 Real-Time PCR System (Email for information)
Services Provided
Qualitative assessment of RNA via Agilent 2100 Bioanalyzer
Affymetrix GeneChip system
- 3' Expression, Exon, Gene, Mapping, Tiling, Promoter or miRNA GeneChips
- Eukaryotic or Prokaryotic Target Preparation
- Whole Transcript, 3" IVT Express or Mapping protocols
- Hybridization of biotinylated, fragmented cRNA to GeneChip
- GeneChip Processing including staining, washing and scanning
- Image analysis of GeneChip and preliminary data processing in Expression or Geneotyping Console
- Data quality assessment using dChip, Partek and BioConductor
Spotted Microarray Services
- Fluorescent Labeling of total RNA or mRNA for spotted microarray hybridization
- Hybridization of labeled fluorescent probes to spotted microarray slides
- Scanning of hybridized spotted microarray slides (Cy3 and Cy5 labeled slides only)
- Image analysis of scanned tiff files with ArrayVision
Data Analysis Software
- Partek Genomics Suite
- Partek Pathway
- dChip
- GeneSpring GT 2
- Ingenuity Pathway Analysis
- TIGR MultiExperiment Viewer
- EASE
- Pathway Express
- HCE
- NetAffx
- IGB
- Agilent 2100 Expert Software - download to review Bioanalyzer .xad files on your own computer.
- ArrayVision v6.0
- CLCbio Genomics Workbench
Robarts Research DNA Sequencing Facility
Equipment
One NanoDrop ND-1000 Spectrophotometer
One Agilent 2100 Bioanalyzer (a generous donation from the Plunkett
Foundation)
One Virtek (Bio-Rad)
Chip Reader
Affymetrix GeneChip System: 2 x Fluidics Stations, 1 x GeneChip Scanner 3000
7G (a generous gift from the Plunkett
Foundation and Manulife Financial)
One Ion Torrent Personal Genome Machine
One Illumina MiSeq
One Covaris S2 for DNA shearing
One Pippin Prep for NGS library construction
Process
Please contact
us to arrange a New User
meeting. A
Project
Request Form must be submitted to the LRGC before any
samples will be processed. Samples are to be delivered to LRGC staff at RRI,
room 4294, between the hours of 8:30-4:30 pm. Samples must be accompanied with
a completed Sample
Submission Form.
Samples are processed on a first come first served basis, please contact the
LRGC for scheduling.
Alternatively, samples can be shipped via courier on dry ice.
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Site designed by Ericka Simon
Maintained by David
Carter
Last update: Wednesday, November 27, 2013