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    Robarts Research Institute

    Welcome to the
    London Regional Genomics Centre

    The London Regional Genomics Centre is a Robarts Research Institute led initiative and has been established through generous support from the Robarts Research Institute, The Schulich School of Medicine and Dentistry at the University of Western Ontario, London Health Sciences Centre, Child Health Research Institute and The London Regional Cancer Program. The facility provides services on a fee-for-service/cost recovery basis and offers Affymetrix GeneChip technologies and NGS services on the Ion Torrent and MiSeq platforms.

     

    Download: Sequence Alignment software comparison

    Download: Pathway Analysis software comparison

    Download Poster: LRGC Services

    Download Poster: LRGC Data QC

    Latest News

    User's Survey (Please email or Fax back to us)

    User's Published Papers (150) (updated December 16, 2014)

     

    Ingenuity Pathway Analysis Online Training

     

    The LRGC now offers Affymetrix CytoScan HD Array

    The LRGC now offers Affymetrix Human Transcriptome Array 2.0

    The LRGC now offers NGS services on the Illumina MiSeq

     

    Now offering the Affymetrix miRNA 4.0 Array

    Now offering Partek Pathway

    Now offering microbiome sequencing Please contact

    Now offering the Affymetrix Mouse Diversity Genotyping Array

     

    CLCBio TV and Tutorials

    Partek Data Analysis Workshop - Slides: NGS and Microarray

     


    Services Provided


    Qualitative assessment of RNA via Agilent 2100 Bioanalyzer

    Affymetrix GeneChip system

    • 3' Expression, Exon, Gene, Mapping, Tiling, Promoter or miRNA GeneChips
    • Eukaryotic or Prokaryotic Target Preparation
    • Whole Transcript, 3" IVT Express or Mapping protocols
    • Hybridization of biotinylated, fragmented cRNA to GeneChip
    • GeneChip Processing including staining, washing and scanning
    • Image analysis of GeneChip and preliminary data processing in Expression or Geneotyping Console
    • Data quality assessment using dChip, Partek and BioConductor

    Data Analysis Software

    Robarts Research DNA Sequencing Facility


    Equipment

    One NanoDrop ND-1000 Spectrophotometer
    One Agilent 2100 Bioanalyzer (a generous donation from the Plunkett Foundation)
    Affymetrix GeneChip System: 2 x Fluidics Stations, 1 x GeneChip Scanner 3000 7G (a generous gift from the Plunkett Foundation and Manulife Financial)
    One Ion Torrent Personal Genome Machine

    One Illumina MiSeq
    One Covaris S2 for DNA shearing

    One Pippin Prep for NGS library construction

     


    Process
    Please contact us to arrange a New User meeting. A Project Request Form must be submitted to the LRGC before any samples will be processed. Samples are to be delivered to LRGC staff at RRI, room 4294, between the hours of 8:30-4:30 pm. Samples must be accompanied with a completed Sample Submission Form.
    Samples are processed on a first come first served basis, please contact the LRGC for scheduling.
    Alternatively, samples can be shipped via courier on dry ice.

     


    Robart's Research Institute

    London Health Sciences Centre
    London Regional Cancer Centre
    Child Health Research Institute
    University of Western Ontario

    Site designed by Ericka Simon

    Maintained by David Carter

    Last update: Tuesday, December 16, 2014