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    		Robarts Research Institute

    Welcome to the
    London Regional Genomics Centre

    The London Regional Genomics Centre is a Robarts Research Institute led initiative and has been established through generous support from the Robarts Research Institute, The Schulich School of Medicine and Dentistry at the University of Western Ontario, London Health Sciences Centre, Child Health Research Institute and The London Regional Cancer Program. The facility provides services on a fee-for-service/cost recovery basis and offers Affymetrix GeneChip technologies and NGS services on the MiSeq and NextSeq platforms.

     

    Download: Sequence Alignment software comparison

    Latest News

    User's Published Papers (239) (updated October 16, 2018)

     

    Now offering Partek Flow for NGS data analysis

    The LRGC now offers Exome sequencing

    The LRGC now offers Affymetrix Clariom D arrays

    The LRGC now offers Affymetrix Clariom S arrays

    The LRGC now offers NGS services on the Illumina NextSeq 500

     

    Now offering microbiome sequencing Please contact

     

    Partek Data Analysis Workshop - Slides: NGS and Microarray

     

    Services Provided


    Qualitative assessment of RNA via Agilent 2100 Bioanalyzer

    Affymetrix GeneChip system

    • 3' Expression, Exon, Gene, Mapping, Tiling, Promoter or miRNA GeneChips
    • Eukaryotic or Prokaryotic Target Preparation
    • Whole Transcript, 3" IVT Express or Mapping protocols
    • Hybridization of biotinylated, fragmented cRNA to GeneChip
    • GeneChip Processing including staining, washing and scanning
    • Image analysis of GeneChip and preliminary data processing in Expression or Geneotyping Console
    • Data quality assessment using dChip, Partek and BioConductor

    Data Analysis Software

    Robarts Research DNA Sequencing Facility

    Equipment

    One NanoDrop ND-1000 Spectrophotometer
    One Agilent 2100 Bioanalyzer (a generous donation from the Plunkett Foundation)
    Affymetrix GeneChip System: 2 x Fluidics Stations, 1 x GeneChip Scanner 3000 7G (a generous gift from the Plunkett Foundation and Manulife Financial)

    One Illumina NextSeq 500
    One Illumina MiSeq
    One Covaris S2 for DNA shearing

    One Pippin Prep for NGS library construction

     

    Process
    Please contact us to arrange a New User meeting. A Project Request Form must be submitted to the LRGC before any samples will be processed. Samples are to be delivered to LRGC staff at RRI, room 4294, between the hours of 8:30-4:30 pm. Samples must be accompanied with a completed Sample Submission Form.
    Samples are processed on a first come first served basis, please contact the LRGC for scheduling.
    Alternatively, samples can be shipped via courier on dry ice.

     

    Robart's Research Institute
    University of Western Ontario

    Site designed by Ericka Simon

    Maintained by David Carter

    Last update: Tuesday, October 16, 2018