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Robarts Research Institute

Quality Control

 

LRGC QA/QC Documents

Due to low inherent background and excellent correlation between technical replicates, we suggest biological, rather than technical, replicates.

 

Mapping 10K v2.0 Quality Statistics - GDAS report.

 

Minimal Background - HGU133A GeneChip hybridized with no labeled cRNA target. This represents an approximate minimal background inherent in the platform.

 

Processing Technical Replicates - Same RNA sample, different processing days. True technical replicates.

 

GeneChip Technical Replicates - Same hybridization cocktail, three separate GeneChips. Assessing chip-to-chip variance.

 

Technical Variance - Same RNA sample, different processing days, different technicians. Assessing variance due to technicians.

 

 

Robart's Research Institute
London Health Sciences Centre
London Regional Cancer Centre
Child Health Research Institute
University of Western Ontario

Site designed by Ericka Simon

Maintained by David Carter

Last update: Sunday, August 22, 2010