LRGC QA/QC Documents
Due to low inherent background and excellent correlation between technical replicates, we suggest biological, rather than technical, replicates.
Mapping 10K v2.0 Quality Statistics - GDAS report.
Minimal Background - HGU133A GeneChip hybridized with no labeled cRNA target. This represents an approximate minimal background inherent in the platform.
Processing Technical Replicates - Same RNA sample, different processing days. True technical replicates.
GeneChip Technical Replicates - Same hybridization cocktail, three separate GeneChips. Assessing chip-to-chip variance.
Technical Variance - Same RNA sample, different processing days, different technicians. Assessing variance due to technicians.
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