Affymetrix Fluidic Station
Affymetrix Fluidic Station
Affymetrix Scanner
BioRad Chip Reader
Viia7
ABI 7900HT
ABI 7900HT
ABI 7900HT

 

  •  

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     


    Robarts Research Institute

    Quality Control

     

    LRGC QA/QC Documents

    Due to low inherent background and excellent correlation between technical replicates, we suggest biological, rather than technical, replicates.

     

    Mapping 10K v2.0 Quality Statistics - GDAS report.

     

    Minimal Background - HGU133A GeneChip hybridized with no labeled cRNA target. This represents an approximate minimal background inherent in the platform.

     

    Processing Technical Replicates - Same RNA sample, different processing days. True technical replicates.

     

    GeneChip Technical Replicates - Same hybridization cocktail, three separate GeneChips. Assessing chip-to-chip variance.

     

    Technical Variance - Same RNA sample, different processing days, different technicians. Assessing variance due to technicians.

     

     


    Robart's Research Institute

    London Health Sciences Centre
    London Regional Cancer Centre
    Child Health Research Institute
    University of Western Ontario

    Site designed by Ericka Simon

    Maintained by David Carter

    Last update: Tuesday, December 1, 2015