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    Robarts Research Institute

    Sample Submission

     

    Total RNA for microarray experiments must be of high quality:

    • 260/280 ratio: 1.8-2.1
    • 260/230 ratio: 1.8-2.1
    • Bioanalyzer electropherogram showing no degradation

    Service Offered

    Experiments

    • A Project Request Form must be submitted before experiments can commence.
    • RNA samples are to be submitted in RNase, DNase free water.
    • Typically 1 week turn around time.
    • Affymetrix GeneChip experiments require the submission of one RNA sample for each GeneChip.

     

    Illumina MiSeq/NextSeq 500

    • DNA-seq (Nextera XT)
      • 1 ng in at most 5 uL RNase, DNase free water
    • RNA-seq (Epicentre)
      • 50 ng rRNA depleted RNA in at most 9 uL RNase, DNase free water

    3' IVT Expression GeneChips

    • Minimum 5 uL of total RNA at 500 ng/uL in RNase, DNase free water.

    Human Transcriptome, Exon and Gene Array GeneChips

    • Minimum 5 uL of total RNA at 100 ng/uL in RNase, DNase free water.

    SNP 6.0, Mouse Diversity Genotyping Arrays

    • Minimum 12uL of genomic DNA at 50ng/uL (required concentration) in reduced EDTA TE buffer (0.1 mM EDTA, 10mM Tris HCl, pH 8.0).

    250K Mapping GeneChips

    • Minimum 6uL (per array) of genomic DNA at 50ng/uL (required concentration) in reduced EDTA TE buffer (0.1 mM EDTA, 10mM Tris HCl, pH 8.0).

    miRNA 4.0 GeneChips

    • Minimum 8 uL of total RNA at 100 ng/uL in RNase, DNase free water.

    Tiling Arrays

    • Promoter (single array): 7.5 ug total purified PCR product in a maximum volume of 39.0 uL of RNase, DNase free water.
    • Tiling 2.0R (7 arrays sets): 27.0 ug total purified PCR product in a maximum volume of 117.0 uL of RNase, DNase free water.

    Agilent 2100 Bioanalyzer Scans

    • Used to assess the quality of total RNA
    • Minimum 3 uL of total RNA at 200 ng/uL in RNase, DNase free water.


    Data and Data Analysis

    • Data can be downloaded from our ftp server or burned to DVD.
    • Computer workstations can be reserved for data analysis. Please sign up in advance.

    Robart's Research Institute

    London Health Sciences Centre
    London Regional Cancer Centre
    Child Health Research Institute
    University of Western Ontario

    Site designed by Ericka Simon

    Maintained by David Carter

    Last update: Tuesday, October 18, 2016