Sample Submission

Total RNA for microarray experiments must be of high quality:

• 260/280 ratio: 1.8-2.1
• 260/230 ratio: 1.8-2.1
• Bioanalyzer electropherogram showing no degadation

Service Offered

Microarray Experiment

• A Project Request Form must be submitted before experiments can commence.
• RNA samples are to be submitted in RNase, DNase free water.
  
• Typically 1 week turn around time.
• Affymetrix GeneChip experiments require the submission of one RNA sample for each GeneChip.
• Two aliquots, one labeled "M" for microarray, a second labeled "B" for Bioanalyzer, of each RNA sample must be submitted in labeled 1.5 ml microcentrifuge tubes.
• The "M" tube will be used for the microarray and the second "B" tube will be used for the Agilent 2100 Bioanalyzer and will contain 5 uL at 200 ng/uL.

3' IVT Expression GeneChips

• Minimum 5 uL of total RNA at 500 ng/uL in RNase, DNase free water.

Exon and GeneArray GeneChips

• Minimum 5 uL of total RNA at 100 ng/uL in RNase, DNase free water.

Cytogenetics Whole-Genome 2.7M Array

• Minimum 6uL of genomic DNA at 33ng/uL (required concentration) in reduced EDTA TE buffer (0.1 mM EDTA, 10mM Tris HCl, pH 8.0).

SNP 5.0/6.0 Mapping GeneChips

• Minimum 12uL of genomic DNA at 50ng/uL (required concentration) in reduced EDTA TE buffer (0.1 mM EDTA, 10mM Tris HCl, pH 8.0).

500K Mapping GeneChips

• Minimum 6uL (per array) of genomic DNA at 50ng/uL (required concentration) in reduced EDTA TE buffer (0.1 mM EDTA, 10mM Tris HCl, pH 8.0).

miRNA GeneChips

• Minimum 8 uL of total RNA at 250 ng/uL in RNase, DNase free water.

Tiling Arrays

• Promoter (single array): 7.5 ug total purified PCR product in a maximum volume of 39.0 uL of RNase, DNase free water.
• Tiling 2.0R (7 arrays sets): 27.0 ug total purified PCR product in a maximum volume of 117.0 uL of RNase, DNase free water.

Agilent 2100 Bioanalyzer Scans

• Used to assess the quality of total RNA
• Minimum 3 uL of total RNA at 200 ng/uL in RNase, DNase free water.

Spotted cDNA Array Scanning and Image Analysis

• BioRad VersArray ChipReader scanner (5 micron resolution)
• Cy3 and Cy5 filters installed
• Standard microscope slide format

Data and Data Analysis

• Data can be downloaded from our ftp server or burnt to DVD.
• Computer workstations can be reserved for data analysis. Please sign up in advance.

Site designed by Ericka Simon

Maintained by David Carter

Last update: Sunday, August 22, 2010