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    Robarts Research Institute

    Sample Submission

     

    DNA/RNA isolation considerations for Illumina Library preparation kits:

    • 260/280 ratio: 1.8 - 2.0
    • 260/230 ratio: 2.0 - 2.2
    • Illumina Recommendations
    • Bioanalyzer electropherogram showing no RNA degradation.

    Services Offered

    Experiments

    • A Project Request Form must be submitted before experiments can commence.
    • RNA samples are to be submitted in RNase, DNase free water.
    • Typically 7-10 day turn around time.

     

    Illumina MiSeq/NextSeq 500

    • DNA-seq (Nextera XT)
      • 1 ng in at most 5 uL RNase, DNase free water
    • RNA-seq
      • 1-5 ug total RNA in at most 28 uL RNase, DNase free water
    • miRNA-seq
      • 500 ng total RNA in at most 5 uL RNase, DNase free water

    Agilent 2100 Bioanalyzer Scans

    • Used to assess the quality of total RNA or size of DNA
    • Minimum 3 uL of total RNA at 200 ng/uL in RNase, DNase free water.


    Data and Data Analysis


    Robart's Research Institute

    University of Western Ontario

    Site designed by Ericka Simon

    Maintained by David Carter

    Last update: Monday, January 7, 2019